Step-2
ICSI Procedure
After the assessment is done and patient is declared medically fit to undergo the treatment, ICSI procedure will begin
EGG RETRIEVAL
Just like a normal IVF procedure, Egg retrieval in ICSI procedure is also done. After ovarian stimulation through medication, and monitoring the ovulation, a trigger injection of human chorionic gonadotropin (hCG) or a GnRH agonist is administered to induce final egg maturation. Approximately 36 hours later, the eggs are retrieved from the ovaries using transvaginal ultrasound-guided aspiration.
SPERM EXTRACTION
Before the ICSI procedure begins, a semen sample is collected from the male partner. If necessary, the sperm sample may undergo processing in the laboratory to isolate motile and morphologically normal sperm from seminal fluid and debris. In cases of difficulty in sperm retrieval, sperm is retrieved through a treatment named TESA.
SPERM INJECTION INTO EGG
The selected sperm is carefully injected directly into the cytoplasm (inner part) of the egg using the micropipette. The embryologist ensures precise positioning of the sperm within the egg to maximize the chances of successful fertilization.
MONITORING EMBRYO
Fertilized eggs (embryos) are cultured in the laboratory for 3 to 5 days, as they develop into multicellular embryos. During this time, embryologists monitor embryo development and quality to select the most viable embryos for transfer.
EMBRYO TRANSFER
One or more selected embryos are transferred into the woman's uterus. The number of embryos transferred depends on factors like the woman's age, embryo quality, and previous IVF treatment history.
Approximately 10-14 days after embryo transfer, a pregnancy test is performed to determine whether implantation has occurred and pregnancy has been achieved. If the test is positive, ongoing prenatal care is initiated to monitor the progress of the pregnancy.