Step-2
IVF Procedure
After the assessment is done and patient is declared medically fit to undergo the treatment, IVF procedure will begin
OVARIAN STIMULATION
The first step in IVF treatment is ovarian stimulation, where fertility medications are administered to stimulate the ovaries to produce multiple eggs for about 8-14 days. Regular monitoring through blood tests and transvaginal ultrasound scans is conducted to assess follicle growth and hormone levels.
EGG RETRIEVAL
Once the ovarian follicles containing mature eggs have reached an optimal size, a trigger injection of human chorionic gonadotropin (hCG) or a GnRH agonist is administered to induce final egg maturation. Approximately 36 hours later, the eggs are retrieved from the ovaries using transvaginal ultrasound-guided aspiration.
SPERM EXTRACTION
Then, the male partner provides a semen sample which is processed in the laboratory to isolate high-quality sperm for fertilization. In cases of male factor infertility or when sperm retrieval techniques are needed, sperm may be obtained through techniques such as TESE/ PESA
FERTILISATION
The retrieved eggs are then fertilized with sperm in the laboratory through either conventional insemination or intracytoplasmic sperm injection (ICSI), where a single sperm is directly injected into each mature egg.
EVALUATION OF EMBRYOS
Fertilized eggs (embryos) are cultured in the laboratory for 3 to 5 days, as they develop into multicellular embryos. During this time, embryologists monitor embryo development and quality to select the most viable embryos for transfer.
EMBRYO TRANSFER
One or more selected embryos are transferred into the woman's uterus. The number of embryos transferred depends on factors like woman's age, embryo quality, and previous IVF treatment history.
Approximately 10-14 days after embryo transfer, a pregnancy test is performed to determine whether implantation has occurred and pregnancy has been achieved. If the test is positive, ongoing prenatal care is initiated to monitor the progress of the pregnancy.